Fig. 6

Rescue of BRCA-1 and ERα expression in sporadic UACC-3199 breast cancer cells with αNF. a Bands are representative baseline immunocomplexes detected by Western blotting for BRCA-1 and AhR protein expression in MCF-7 and UACC-3199 breast cancer cells cultured respectively in control phenol-red free DMEM or RPMI 1640 media supplemented with 10 % charcoal-stripped FCS; b UACC-3199 breast cancer cells were cultured in control phenol-red free RPMI plus 10 % charcoal-stripped FCS in the absence (control) or presence of αNF (2 μM for 72 h). Bars represent means ± SEM of quantitation of mRNA (fold change of control) performed twice in duplicate (n = 4) with four repeated measures/sample. BRCA-1 mRNA was corrected for GAPDH mRNA as internal standard; c Bands are immunocomplexes detected by Western blotting for BRCA-1 and ERα in UACC-3199 breast cancer cells cultured in phenol-red free RPMI plus 10 % charcoal-stripped FCS in the absence (control) or presence of αNF (2 μM for 72 h). GAPDH bands are internal standards for Western blotting; d Bars represent means ± SEM of quantitation of CYP1A1 and CYP1B1 mRNA (fold change of control) performed twice in duplicate (n = 4) with four repeated measures/sample, and corrected for GAPDH mRNA as internal standard. In (b) and (d) Asterisks represent statistical differences (P < 0.05) compared to control