Fig. 5

Effect of COX inhibitors and prostasin on matriptase expression. a & b RT-qPCR (n = 3). The UROtsa cells were treated with different dosages of ibuprofen for 24 h or at 2 mM ibuprofen for different time periods. c RT-qPCR (n = 3). The UROtsa cells were treated with COX inhibitors for 24 h. IBU: ibuprofen, 2 mM; COX-1(−): COX-1 inhibitor, 10 μM; COX-2(−): COX-2 inhibitor, 10 μM. a, b, and c The numbers above each bar indicate the fold change of the expression as compared to the DMSO control. ANOVA: p > 0.05. Note: the bars of DMSO and IBU in (c) are the same of those in (a). d Western blot analysis of matriptase in UROtsa treated with different dosages of ibuprofen for 24 h as indicated. Note the samples on the membrane were blotted with the anti-matriptase antibody after blotting with anti-COX-2 and anti-tubulin antibodies shown in Fig. 2b. The previously blotted COX-2 protein bands is shown along with the matriptase protein bands. e Western blot analysis of prostasin, matriptase, HAI-1 expression in stable or transient prostasin over-expressing cell lines as indicated. Vec: vector control; Pro: prostasin over-expressing lines