MicroRNA Expression in Formalin-fixed Paraffin-embedded Cancer Tissue: Identifying Reference MicroRNAs and Variability

Table 1 Optimal number of reference miRNAs – colorectal cancer

Number of miRNAs	V^a	ΔV^a	miRNAs added	Stability increased?
2	–	–	miR-103a-3p, miR-152-3p	–
3 vs 2	0.3475	–	miR-132-3p	–
4 vs 3	0.1400	-0.2075	miR-27a-3p	yes
5 vs 4	0.1728	0.0328	miR-140-5p	no
6 vs 5	0.1145	-0.0583	miR-30b-5p	yes
7 vs 6	0.1189	0.0044	miR-339-5p	no
8 vs 7	0.1005	-0.0185	miR-331-3p	yes
9 vs 8	0.0991	-0.0014	miR-374a-5p	yes
10 vs 9	0.0778	-0.0212	miR-652-3p	yes
11 vs 10	0.0689	-0.0089	miR-335-5p	yes
12 vs 11	0.0728	0.0040	miR-185-5p	no
13 vs 12	0.0588	-0.0140	miR-151-5p	yes
14 vs 13	0.0614	0.0025	miR-106b-5p	no
15 vs 14	0.0503	-0.0111	miR-199a-3p	yes
16 vs 15	0.0574	0.0071	miR-425-5p	no
17 vs 16	0.0426	-0.0147	miR-26a-5p	yes
18 vs 17	0.0685	0.0259	miR-24-3p	no

MiRNAs in bold are the ones that are included in the optimal number of references to use.
^aThe stability measure V is calculated according to Vandesompele et al. [21]

ISSN: 1471-2407