Fig. 5

Treatment of monocyte-derived cells with CD105⁺ EVs induced a release of sHLA-G. a Supernatants were harvested to detect sHLA-G production by ELISA, after 7 days of culture of monocyte-derived cells stimulated with EVs shed by renal cancer cells (CD105⁺ CSCs and CD105^- TCs). Results were obtained from 3 independent experiments and expressed as mean ± SD. ANOVA with Newman Keuls multicomparison test was performed: ** p < 0.001 CD105+ EV Mo versus all the other conditions. b Representative Western Blot analysis showing the presence of HLA-G and Alix within EVs. Hsp90 was used as normalization. Four experiments were performed with similar results