Fig. 6

Total Orai1 abundance in MCF-7 cells following mAR activation in absence and presence of cytochalasin B and Rac1 inhibitor NSC23766. a-e Original histogram of anti-Orai1 fluorescence in permeabilized MCF-7 cells without (a) and with (b-e) a 60 min treatment with testosterone-albumin-conjugates (TAC, 100 nM) in the absence (b) and presence of flutamide (c), cytochalasin B (d) and Rac inhibitor (e). f Arithmetic means ± SEM (n = 6) of the Orai 1 protein abundance in permeabilized MCF-7 cells without (white bar) and with a 15 min to 24 h treatment with testosterone-albumin-conjugates (TAC, 100 nM) in the absence (black bars) and presence of flutamide (1 μM) (dark grey bars) cytochalasin B (1 μM) (middle grey bars), or Rac inhibitor NSC23766 (50 μM) (light grey bars). ***(p < 0.001) indicates statistically significant difference from absence of TAC, ###(p < 0.001) indicates statistically significant difference from presence of TAC without presence of inhibitors