Fig. 4

Effect of mAR activation on abundance of activated Rac1 protein in MCF-7 cells. a Affinity precipitation with GST (glutatione S-transferase) -PBD (p21 binding domain) revealing by immunoblotting (IB) the protein abundance of activated (upper lane) and total (lower lane) Rac1 prior to (control) and 15–120 min following treatment with testosterone-albumin-conjugates (TAC, 100 nM) and TAC + Rac1 inhibitor NSC23766 (50 μM). b Arithmetic means ± SEM (n = 4) of the relative fold increases of activated over total Rac1 protein abundance prior to (taken as 1) and 15–120 min following treatment with testosterone-albumin-conjugates (TAC, 100 nM) and TAC + Rac1 inhibitor NSC23766 (50 μM). **(p < 0.01) indicates statistically significant difference from absence of TAC