Fig. 5

Reversible in vivo action. NCI-H716 tumors were isolated after drug treatment (TP1) and at the end of the experiment (TP2). a Effect of the FGFR inhibitor on proliferation, hypoxia, necrosis, apoptosis and micro vessel density (MVD). b Comparison between TP1 and TP2 (= effect drug cessation). Columns indicate mean ± STDEV of at least 20 tumor sections per treatment group. ∗Significantly different from one another (p < 0.05; two-tailed student’s t-test). c Western blot for indicated proteins. β-actin served as loading control. Shown blots are from three tumors from different mice per group. d mRNA expression in isolated tumors at TP1. e Comparison of mRNA expression levels between TP1 and TP2. Data = means ± SEM of three independent experiments. ∗Significantly different from each other (p < 0.05; two-tailed student’s t-test)