Table 2 Characteristics of the primers used for RT-qPCR
Gene symbol | Targeted transcript variants | Primer sequences (5′ – 3′) | Amplicon (bp) |
|---|---|---|---|
TGFB1 | NM_000660 | (F): TCGCCAGAGTGGTTATCTT | 148 |
(R): TAGTGAACCCGTTGATGTCC | |||
TGFB2 | NM_001135599, NM_003238 | (F): ACACTCAGCACAGCAGGGTCCT | 80 |
(R): TTGGGACACGCAGCAAGGAGAAG | |||
TGFB3 | NM_003239 | (F): TGAGTGGCTGTTGAGAAGAGA | 139 |
(R): ATTGTCCACGCCTTTGAATTTGAT | |||
TGFBR1 | NM_001130916, NM_004612 | (F): GCAGAGCTGTGAAGCCTTGAGA | 128 |
(R): TGCCTTCCTGTTGACTGAGTTG | |||
TGFBR2 | NM_001024847, NM_003242 | (F): ATGACATCTCGCTGTAATGC | 163 |
(R): GGATGCCCTGGTGGTTGA | |||
HPRT1 | NM_000194 | (F): TGGCGTCGTGATTAGTGATG | 160 |
(R): GCCTCCCATCTCCTTCATC | |||
GAPDH | NM_001256799, NM_002046 | (F): TCTCTGCTCCTCCTGTTC | 112 |
(R): CGACCAAATCCGTTGACT |