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Fig. 2 | BMC Cancer

Fig. 2

From: Placental growth factor inhibition modulates the interplay between hypoxia and unfolded protein response in hepatocellular carcinoma

Fig. 2

PlGF inhibition reduces intratumour hypoxia in experimental HCC. a Immunostaining for pimonidazole in mouse livers following the indicated treatment. Arrows indicate tumours. Scale bars: 100 μm. b Quantification of the immunostaining for pimonidazole. c Lysates of control liver tissue or isolated DEN-induced tumours were subjected to Western blotting for detection of pimonidazole adducts (Pimo). Blotting of β-actin is shown as a loading control. d Densitometry analysis of the pimonidazole blot in (c). e Real-time PCR analysis of Glut1 and Pfk mRNA levels in tumour tissues. IgG = 25w DEN + 5w IgG, aPlGF = 25w DEN + 5w aPlGF. Data are presented as the means ± SD. *p < 0.05

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