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Fig. 1 | BMC Cancer

Fig. 1

From: Silvestrol induces early autophagy and apoptosis in human melanoma cells

Fig. 1

Silvestrol inhibits cell proliferation in MDA-MB-435 human melanoma cells. a Cells were exposed to silvestrol at 25 nM for the indicated times and evaluated for survival by the MTS assay. b Immunoblot analysis of cyclin B1 and cyclin D1. Cells treated with DMSO and different doses of silvestrol for 24 h and harvested. Cell lysates were resolved in SDS-PAGE and probed with specific antibodies against cyclin B1, cyclin D1 and β-actin. c Silvestrol inhibits cell proliferation by inducing G2-phase accumulation. Cells were treated with DMSO or silvestrol for 24 h prior to analysis by flow cytometry. d Graph displaying differences in cell cycle phases. Data represented as means ± SEM, * p ≤0.05

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