Fig. 2

Sulindac sulfide inhibits colon cancer cell growth and decreases expression of Sp1, Sp3 and Sp4 proteins. a, c Sulindac sulfide inhibits SW480 and RKO cell proliferation. Cells were treated with DMSO, 25, 50, and 75 μM sulindac sulfide for 24 and 48 h. Cell numbers were determined as described under Materials and Methods. Experiments were done in triplicate and results are expressed as percentage of control (mean ± S.D.). b, d Sulindac sulfide decreases expression of Sp1, Sp3 and Sp4 proteins in SW480 and RKO cells. Cells were treated with DMSO, 25, 50, 75 and 100 μM sulindac sulfide for 24 and 48 h. Sp1, Sp3 and Sp4 cellular levels were determined by Western blot analysis as described under Materials and Methods and β-actin was used as loading control