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Fig. 4 | BMC Cancer

Fig. 4

From: TSG101, a tumor susceptibility gene, bidirectionally modulates cell invasion through regulating MMP-9 mRNA expression

Fig. 4

TSG101 depletion has little impact on the signaling pathways in PMA-stimulated HT1080 cells. Cells transfected with control (con) or TSG101 (TSG#1) siRNA were incubated in fresh serum-free medium containing the indicated reagents. ab. Cells were incubated with 50 μM PD98059, 10 μM SB203580, 50 μM SP600125, or 50 μM LY294002 in fresh serum-free medium containing or not 200 nM PMA for 7 h. MMPs in conditioned media from PMA-treated (a) and non-treated (b) cells were measured using gelatin zymography. c. Cells were incubated in fresh serum-free medium containing 200 nM PMA for the indicated periods of time. Cell lysates were analyzed by western blot using the indicated antibodies. The blots and gels shown are representative of three independent experiments

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