Fig. 4
From: Gonadotropin-mediated chemoresistance: Delineation of molecular pathways and targets
Down-modulation of SURVIVIN, HIF-1α, HO-1 and NRF-2 decreases hCG-induced chemo-resistance. a mRNA levels by semi-quantitative RT-PCR (left panel) and protein levels by Western blot (right panel) of SURVIVIN, HIF 1-α, HO-1 and NRF-2 upon transfection of respective siRNAs in ChaGo-K-1 cells. scRNA: scrambled RNA control. β-ACTIN was employed as loading control. b-e Cell viability analysis of ChaGo-K-1 cells individually transfected with siRNAs against SURVIVIN, HIF 1-α, HO-1 and NRF-2 incubated with hCG and later exposed to (b, d) tamoxifen or (c, e) curcumin. scRNA: scrambled RNA control. For b-e, means ± SEM of four independent experiments (with three replicates) are shown. In each case, data has been individually normalized to control cultures. (&p < 0.0003 vs drug + hCG; *p < 0.003, #p < 0.0003, $p < 0.03 vs hCG + respective scRNA + respective drug)