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Table 1 Size and number of alleles from short tandem repeat (STR) analysis

From: Macrophage traits in cancer cells are induced by macrophage-cancer cell fusion and cannot be explained by cellular interaction

STR marker Hybrids Macrophages MCF7 cells
AMEL 105, 110/2 105, 110/2 105/1
DXYS218 323, 327, 331/3 327, 331/2 327/1
SRY 204/1 204/1 -
X22 205, 209, 224/3 205, 224/2 209/1
XHPRT 277, 289/2 277/1 289/1
DXS6803 123, 126/2 126/1 123/1
DXS6854 107/1 107/1 107/1
D13S305 449/1 445, 456/2 448/1
D13S628 455/1 456/1 455/1
D13S634 394, 399/2 394/1 399/1
D13S742 287/1 262, 290/2 286/1
D13S797 196, 200/2 196/1 196, 200/2
D18S386 351, 355, 388, 392/4 355, 388 /2 351, 392/2
D18S390 338, 342/2 342/1 338, 342/2
D18S391 160/1 160/1 160/1
D18S535 475, 483, 487/3 475, 487/2 483/1
D18S819 251, 254, 262/3 251, 254/2 262/1
D18S976 185/1 186, 189/2 185/1
D21S11 253, 267/2 245, 255/2 253, 267/2
D21S1246 293, 297, 318/3 293, 318/2 293, 297/2
D21S1409 203, 210, 214/3 210, 214/2 203, 214/2
D21S1411 307, 312/2 299, 332/2 307, 312/2
D21S1435 375, 383, 387/3 375, 383/2 375, 387/2
D21S1444 314, 318/2 318/1 314, 318/2
  1. STR analysis was used to determine the DNA profile of M macrophages, GFP-labeled MCF-7 breast cancer cells, and MCF-7/macrophage hybrids. A total of 24 loci on X and Y chromosomes as well as chromosomes 13, 18 and 21 were used in this analysis. The macrophages originated from M2-activated monocytes harvested from male blood donors and contained Y chromosome. MCF-7 is a breast cancer cell line isolated originally from a female patient. Of 24 markers, 17 were of the same allelic size in MCF-7/macrophage hybrids. For several loci at least one allele was common to macrophages and MCF-7 cancer cells represented in the hybrids. Sex-determining region Y gene (SRY) is one of the markers found in MCF-7/macrophage hybrids, indicating that these cells originate from fusion between macrophages and MCF-7 cells. Note that the hybrids contain the same size of alleles on the X-chromosome markers as do macrophages and MCF-7 cells