|
STR marker
|
Hybrids
|
Macrophages
|
MCF7 cells
|
|---|
|
AMEL
|
105, 110/2
|
105, 110/2
|
105/1
|
|
DXYS218
|
323, 327, 331/3
|
327, 331/2
|
327/1
|
|
SRY
|
204/1
|
204/1
|
-
|
|
X22
|
205, 209, 224/3
|
205, 224/2
|
209/1
|
|
XHPRT
|
277, 289/2
|
277/1
|
289/1
|
|
DXS6803
|
123, 126/2
|
126/1
|
123/1
|
|
DXS6854
|
107/1
|
107/1
|
107/1
|
|
D13S305
|
449/1
|
445, 456/2
|
448/1
|
|
D13S628
|
455/1
|
456/1
|
455/1
|
|
D13S634
|
394, 399/2
|
394/1
|
399/1
|
|
D13S742
|
287/1
|
262, 290/2
|
286/1
|
|
D13S797
|
196, 200/2
|
196/1
|
196, 200/2
|
|
D18S386
|
351, 355, 388, 392/4
|
355, 388 /2
|
351, 392/2
|
|
D18S390
|
338, 342/2
|
342/1
|
338, 342/2
|
|
D18S391
|
160/1
|
160/1
|
160/1
|
|
D18S535
|
475, 483, 487/3
|
475, 487/2
|
483/1
|
|
D18S819
|
251, 254, 262/3
|
251, 254/2
|
262/1
|
|
D18S976
|
185/1
|
186, 189/2
|
185/1
|
|
D21S11
|
253, 267/2
|
245, 255/2
|
253, 267/2
|
|
D21S1246
|
293, 297, 318/3
|
293, 318/2
|
293, 297/2
|
|
D21S1409
|
203, 210, 214/3
|
210, 214/2
|
203, 214/2
|
|
D21S1411
|
307, 312/2
|
299, 332/2
|
307, 312/2
|
|
D21S1435
|
375, 383, 387/3
|
375, 383/2
|
375, 387/2
|
|
D21S1444
|
314, 318/2
|
318/1
|
314, 318/2
|
- STR analysis was used to determine the DNA profile of M macrophages, GFP-labeled MCF-7 breast cancer cells, and MCF-7/macrophage hybrids. A total of 24 loci on X and Y chromosomes as well as chromosomes 13, 18 and 21 were used in this analysis. The macrophages originated from M2-activated monocytes harvested from male blood donors and contained Y chromosome. MCF-7 is a breast cancer cell line isolated originally from a female patient. Of 24 markers, 17 were of the same allelic size in MCF-7/macrophage hybrids. For several loci at least one allele was common to macrophages and MCF-7 cancer cells represented in the hybrids. Sex-determining region Y gene (SRY) is one of the markers found in MCF-7/macrophage hybrids, indicating that these cells originate from fusion between macrophages and MCF-7 cells. Note that the hybrids contain the same size of alleles on the X-chromosome markers as do macrophages and MCF-7 cells
