Fig. 4

Dox activated AKT signaling pathway in human TCC cells in dose- and time-dependent manner. a T24 and UMUC3 cells were treated with 0.1, 0.5 and 1 μM Dox and AD198 for 24 h. Protein levels of p-AKT (T308), p-AKT (S473), AKT, p-GSK-3β, p-ERK1/2 and ERK1/2 were detected by WB. Actin was used as a loading control. b T24 and UMUC3 cells were treated with 1 μM Dox and AD198 for 0, 0.5, 1, 3, 6 and 24 h. Protein levels of p-AKT (T308), p-AKT (S473), AKT, p-p38, p38, p-ERK1/2 and ERK1/2 were detected by WB. Actin was used as a loading control