Table 3 Coexisting somatic mutations resulting in amino-acid changes identified using the Ion AmpliSeq Hotspot Panel version 2
| Â | Â | Frequency (%) | Â | Frequency (%) | Â | Frequency (%) | Â | Frequency (%) | Â | Frequency (%) |
|---|---|---|---|---|---|---|---|---|---|---|
Case 1 | KIT M541L (COSM 28026) | 70.9 | TP53 P72R | 53.2 | --- | --- | --- | --- | --- | --- |
Case 2 | PTEN L57W (COSM 5253) | 21.2 | --- | --- | --- | --- | --- | --- | --- | --- |
Case 3 | TP53 P72R | 57.0 | CTNNB1 D32N (COSM 5672) | 34.5 | TP53 V73 del | 29.1 | CDH1 Q346* (COSM 19524) | 25.1 | --- | --- |
Case 4 | TP53 P72R | 60.3 | TP53 R337C (COSM 11071) | 18.0 | --- | --- | --- | --- | --- | --- |
Case 5 | TP53 P72R | 46.9 | KDR Q472H | 46.9 | KIT G534C | 46.3 | APC S1463fs | 42.5 | --- | --- |
Case 6 | PDGFRA P567Q | 100 | TP53 V73W | 72.6 | TP53 P151S | 57.5 | KDR Q472H | 42.4 | ERBB4 C614Y | 38.2 |
| Â | SMAD4 R189H | 29.0 | PTEN R233Q | 18.5 | APC D1591N | 18.4 | HRAS T64* | 17.9 | AKT1 T21I | 16.4 |
| Â | KIT L647F | 16.2 | SKT11 D352N | 15.1 | PTEN H123Y (COSM 5078) | 7.3 | PTEN R130Q (COSM 5033) | 7.2 | --- | --- |
Case 7 | TP53 P72R | 96.7 | SKT11 F345L | 53.5 | SKT11 P281L | 53.3 | KDR Q472H | 42.6 | --- | --- |
Case 8 | TP53 P72R | 98.4 | KIT M541L (COSM 28026) | 59.8 | TP53 V154G (COSM 43903) | 35.4 | KDR Q472H | 26.7 | SMAD4 G423R | 14.7 |
| Â | ABL1 I347fs | 11.1 | ERBB4 C759T | 8.8 | FBXW7 M467I | 8.0 | MLH1 A169V | 8.0 | KDR G1284R | 7.9 |
| Â | APC P1433L | 6.7 | TP53 F338L | 6.5 | SMO P610S | 6.4 | MET D340A | 5.8 | NOTCH1 V1575M | 5.7 |
| Â | PTEN A328E | 5.6 | APC G1374K (COSM 18737) | 5.1 | MLH1 R148W | 5.0 | --- | --- | --- | --- |
Case 9 | APC E1464fs | 59.2 | TP53 P72R | 48.2 | BRAF G442D | 6.1 | MET G1102D | 5.5 | SMO T223I | 5.0 |
Case 10 | MET N375K | 55.7 | TP53 P72R | 42.0 | CTNNB1 G34V | 6.5 | --- | --- | --- | --- |
Case 11 | TP53 P72R | 68.5 | PTEN N329fs (COSM 4932) | 39.5 | TP53 K132R (COSM 11582) | 29.7 | --- | --- | --- | --- |
Case 12 | TP53 P72R | 98.1 | KDR Q472H | 96.4 | TP53 V272fs | 21.0 | RB1 I682T | 12.6 | APC P1433L | 9.6 |
| Â | RET E884V | 9.1 | SMAD4 V354L | 8.0 | --- | --- | --- | --- | --- | --- |
Case 13 | TP53 P72R | 99.1 | CDKN2 G155S | 51.6 | FLT3 W603* | 45.2 | KRAS E37K | 33.3 | SMO P641L | 23.7 |
| Â | IDH1 L103M | 20.0 | TP53 R267Q (COSM 43923) | 18.8 | GNA11 D205N | 16.2 | SMARCB1 P165S | 14.0 | RB1 M761T | 13.9 |
| Â | SMARCB1 V145L | 12.4 | TP53 G245R (COSM 10957) | 10.8 | NOTCH1 H1591T | 10.7 | ERBB4 G240V | 10.0 | KIT S715N | 9.9 |
| Â | FBXW7 R505H (COSM 25812) | 9.8 | FBXW7 M498I | 9.2 | MET S186L | 8.8 | IDH1 A111V | 8.8 | JAC3 V133I | 8.5 |
| Â | KIT V825I (COSM 19110) | 8.1 | TP53 G112S | 6.5 | TP53 K132E (COSM 10813) | 6.3 | HNF1A A193V | 6.3 | VHL K171T | 5.7 |
| Â | ALK P1191A | 5.6 | HNF1A T204I | 5.3 | --- | --- | --- | --- | --- | --- |
Case 14 | PTEN H1047L | 62.9 | FGFR3 R765S | 7.2 | IDH1 P118L | 5.7 | --- | --- | --- | --- |
Case 15 | TP53 P72R | 100 | MET A179M | 5.1 | --- | --- | --- | --- | --- | --- |