Fig. 4

Effects of NRP2 on TGF-β Signaling and synergistic effects on migration. a Knock-down of NRP2 does not affect phosphorylation of Smad2 (pSmad2) in SNU-449 cells after treatment with 2.5 ng/mL TGF-β1 for 6 h. Actin was used as loading control. b Translocation of Smad2/3 into the nucleus of SNU-449 cells treated with control siRNA (siNT) or siRNA against NRP2 (siNRP2) after treatment with 2.5 ng/mL TGF-β1 for 1 h. c Transwell assays of SNU-449 cells assessing the migratory impact of reduced TGF-β signaling by LY2109761 (LY) and diminished NRP2 expression by knock-down (siNRP2), either alone or in combination. The treatment of cells with non-target siRNA (siNT) was set to 100 %. Error bars depict SD from 3 independent experiments that were carried out in triplicates. ***, p < 0.001