Fig. 5

Cleaved caspase-3 and Annexin V/PI apoptosis assays. a Cal-78 and SW-1353 cells were treated with 300 μM diacerein. Cleavage of caspase-3 was detected after 48 h by flow cytometry. The y-axis denotes cell counts and the x-axis represents fluorescence intensity of APC antibody. Black filled histogram represents untreated control cells, striated histogram represents 30 μM diacerein, blue lanes showed 100 μM diacerein, and margenta lanes showed 300 μM diacerein treated cells. Green lanes represented the Staurosporin positive control. b Likewise, AnnexinV/PI staining under the influence of 300 μM diacerein for 48 h confirmed the lack of apoptosis induction. c In Cal-78 cells, the DNA damage marker H2AX was marginally increased. β-actin was used as loading control