Fig. 2

Daidzein induces BRF1 and BRF2 protein specifically in MCF-7 cells. MCF-7 (a) and MDA-MB-231 (b) cells were treated with 0 or 3 μM daidzein for 48 h and then analysed for expression of BRF1, BRF2 and actin proteins by western blot. Quantification of BRF2 protein expression in (c) MCF-7 and (d) MDA-MB-231 cells treated with daidzein was achieved by ELISA. Data presented are average of four independent experiments. Statistical analysis was performed using one-way ANOVA with a Tukey’s post-test with a 95 % confidence interval (Graphpad Prism 3.03); * = p <0.05; ** = p < 0.01; *** = p < 0.001