Fig. 1
From: Using second harmonic generation to predict patient outcome in solid tumors
Methodology diagrams. a A depiction of the forward- and backward-propagating SHG signal. Red excitation light is focused into the sample by objective lens 1, then SHG is emitted in the backwards direction (towards lens 1) or the forward direction (towards lens 2). b A flowchart of the methodology used to analyze SHG images and calculate the F/B ratio. c An F/B image of one patient sample. Scale bar is 50 μm