Fig. 4

Effects of NF-κB and/or mTOR acidity on miR-223 expression. a Effects of NF-κB inhibitor and/or mTOR inhibitor on miR-223. MCF-7 or PC3 cells were treated with NF-κB inhibitor (PDTC) and/or mTOR inhibitor Ku-0063794 (abbreviated as Ku) for 1 or 6 h, then, the RNA (6 h samples) was extracted and miR-223 determined by quantitative reverse-transcription PCR. Protein (for 1 h) was extracted and phosphorylated NF-κB and mTOR were determined by Western blot. b Effects of mTOR inhibitor on celastrol-induced miR-223 expression. Cells were treated with celastrol and/or mTOR inhibitor for 6 h, then the miR-223 was detected. Cel represents celastrol. Tests were repeated three times. Data are presented as mean ± SD; * indicates P < 0.05, while ** indicates P < 0.01