Fig. 6

NF-κB and Akt pathways may be involved in the anti-cancer effects of L2H17. CT26.WT cells were treated with L2H17 (10 μM) for indicated times (0, 10, 30, 60, 120, or 240 min, a & b) or treated with indicated concentrations of L2H17 (1, 3, 10, or 30 μM, c & d) for 30 min. Then the total protein was extracted and the expression of IκB-α (a,c), p-Akt (b,e) proteins were detected by Western blot analysis. (d) CT26.WT cells were treated with L2H17 (10 μM) or vehicle control (DMSO) for 1 h. Then the cells were harvested and lysed, nuclear extracts were prepared, and p65 expression levels in the nucleus were measured by Western blot assay. The column figures show the normalized optical density from the data from three independent experiments. Data are presented as the mean ± SEM. The indicated differences are significant * P <0.05, ** P < 0.01, and *** P < 0.001, t-test, compared to the DMSO-treated group