Fig. 5

L2H17 inhibited cell migration and invasion of CT26.WT cells. CT26.WT cells were seeded and allowed to adhere overnight. The next day, the “reference line” was drawn at the bottom of each plate. After washed with PBS, the cells were treated with L2H17 (10 μM), curcumin (10 μM) or DMSO (vehicle control) in the presence of mitomycin C for indicated times (24 or 48 h). (a) Representative photomicrographs are shown from one out of four independent cell migration experiments. On 0 h images, the “reference line” was drawn to represent the starting points for CT26.WT cells. (b) The mobility of CT26.WT cells was calculated for L2H17- or curcumin-treated groups as compared with the DMSO-treated (vehicle control) group for each experiment in different fields. The bar chart represents means (± SEM) of four independent experiments. CT26.WT cells were seeded in the transwell plate that was coated with Matrigel. Then the cells were treated with either L2H17 (1, 3, or 10 μM) or curcumin (1, 3, or 10 μM) for 24 h. And then the invasion cells were fixed, stained and viewed under a light microscope (×100; Nikon, Tokyo, Japan). (c) Representative photomicrographs of DMSO control (vehicle control), L2H17- and curcumin-treated invading cells are shown from one out of four independent. (d) The numbers of invading cells in L2H17- or curcumin-treated groups as compared with the DMSO-treated (vehicle control) group for each experiment in at least 15 representative microscopic fields. (e) CT26.WT cells were treated with L2H17 (10 μM) for 12 h. Then the total protein was extracted and the expression of E-cadherin were detected by Western blot analysis. The column figures show the normalized optical density from the data from three independent experiments. The bar chart represents means (± SEM) from one out of three independent experiments. The indicated differences are significant *P <0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, t-test, L2H17-treated compared to the DMSO-treated group; #P <0.05, ##P < 0.01, ###P < 0.001, and ####P < 0.0001, t-test, curcumin-treated compared to the DMSO-treated group