Fig. 3

L2H17 induced cell cycle arrest and apoptosis in CT26.WT cells. (a) CT26.WT cells were treated with L2H17 (1, 3, 10, or 30 μM) or curcumin (30 μM) for 24 h, and the cell cycle distribution was analyzed by the cytometry (Becton Dickinson FACSCalibor, BD Biosciences, Franklin Lakes, NJ). The data were obtained from four independent experiments performed in triplicate. And the representative histogram of the cell cycle distribution was shown. (b) The mean of the four values was calculated, and the percentages of cells in Sub-G0/G1, S and G2/M phases are shown. (c) The effects of L2H17 and curcumin on the induction of apoptosis in CT26.WT cells. CT26.WT cells were treated with DMSO or the indicated concentration of L2H17 (1, 3, 10, or 30 μM) or curcumin (30 μM) for 48 h, and then stained with Annexin V and PI, followed by detection using flow cytometry. The data were obtained from four independent experiments performed in triplicate. Representative data are shown (c), and the percentages of cells with early apoptosis (d) are shown. Data are presented as the mean ± SEM. The indicated differences are significant * P <0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, t-test, compared to the DMSO-treated group