Fig. 5

CIP2A modulate JNK signaling pathway and transcriptional activity. a Western-blots showed the phosphorylation changes of MKK4/7, JNK, c-Jun and ATF2 in association with the expression change of CIP2A in H460 and H838. Densitometry analysis performed in Image J showed the folds of phosphorylation change. The phosphorylation changes were normalized to non-phosphorylated form which was normalized with actin. b The luciferase activity of the downstream target of JNK, AP-1, was measured in H460 and H838 with overexpression or knock down of CIP2A. c Cell proliferation assay of H460 and H460 overexpressing CIP2A cell lines in the presence or absence of JNK inhibitor, SP10025