Fig. 4

MiR-363 targets the 3’ UTR of MYO1B a Schematic representation of the 3’UTR of MYO1B with two complementary miR-363 target sites. Below, mature miR-363 sequences aligned to target sites as predicted by TargetScan, miRanda, and Diana. b MiR-363 targets binding site 1 within the MYO1B 3’-UTR. Four constructs were created that contained the wild-type MYO1B 3’ UTR, 3’ UTR with BS1 or BS2 deletion, and 3’ UTR with both BS1 and BS2 deletions, cloned downstream of the firefly luciferase gene in the pMiR-REPORT™ vector. Each of the constructs were transfected separately into JHU028 cells. MiR-363 significantly reduced firefly luciferase activity of pMiR-REPORT™ plasmid containing the wild-type MYO1B 3’ UTR or a plasmid lacking the BS2, implicating miR-363 binds to MYO1B 3’-UTR BS1 to suppress MYO1B expression; *p < 0.001 when compared to WT MYO1B 3’UTR