Network analysis of an in vitro model of androgen-resistance in prostate cancer

BMC Cancer

Table 1 Changes in the expression of individual isoforms of the Androgen Receptor in our cell line model and in the human tumour data. No significant differential expression is seen in either dataset. Transcripts are taken from the Ensembl AR Gene Transcript Table (ENSG00000169083)

Transcript Identifier	HGNC transcript name	Protein length	Domains	Cell Line Log Fold-Change	Cell Line P-Value	Tumour Log Fold-Change	Tumour P-Value
ENST00000374690	AR-001	920aa	NTD, ZF, LBD	0.03	0.95	0	1
ENST00000396043	AR-002	388aa	ZF, LBD	0	1	−0.11	0.92
ENST00000513847	AR-003^	-	-	0.72	1	0	1
ENST00000504326	AR-004 (AR-V7)	644aa	NTD, ZF	−1.38	0.57	1.82	0.69
ENST00000514029	AR-005*	600aa	NTD, ZF(partial)	−0.19	1	0	1
ENST00000396044	AR-201	734aa	ZF	0	1	−0.08	0.96
ENST00000612010	AR-202	642aa	NTD, ZF	ND	ND	ND	ND
ENST00000612452	AR-203	737aa	NTD (partial), ZF, LBD	ND	ND	ND	ND
ENST00000613054	AR-204	572aa	NTD	ND	ND	ND	ND

*Nonsense mediated decay; ^Processed transcript; ND not detected, NTD N-terminal domain, ZF zinc finger, LBD ligand binding domain. AR-004 is identified as the AR-V7 transcript based on the description of the isoform provided by Krause and colleagues (2014), as the protein encoded by this transcript aligns to the first 627aa of the full length AR, and contains 15 unique amino acids, with one overlapping a splice site [19]

ISSN: 1471-2407