Fig. 3
From: Mice null for the deubiquitinase USP18 spontaneously develop leiomyosarcomas
Leiomyosarcoma cell lines are aneuploid and overexpress MYC. a and b: Cytogenetic analyses showed that both KHC-1 and KHC-2 had polysomy of chromosomes 3 and 15 (black arrows). c: Immunoblot analysis of MYC protein as shown for KHC-1 and KHC-2 cell lines as compared to NIH-3T3L1 and mouse embryonic fibroblast (MEF) cells. d: Immunoblot analysis of MYC levels validated knockdown by two independent siRNAs in KHC-1 and KHC-2 cells. e and f: Analysis of cell growth in KHC-1 and KHC-2 cells transfected with MYC or control siRNAs