Development of a fluorescence–based multiplex genotyping method for simultaneous determination of human papillomavirus infections and viral loads

Table 3 Analyses of discordant HPV typing results between the BMRT PCR assay and sequencing by comparing to the historical diagnoses and infection states of the cases

Type	Numbers of BMRT negative/sequencing positive			Numbers of BMRT positive/sequencing negative
	Infection states		Historical diagnoses	Infection states		Historical diagnoses
	Total	(Single + multiple^a)	(N + CINI+ CINII-III + cancer)	Total	(Single + multiple^a)	(N + CINI+ CINII-III + cancer)
HPV16	3	(2 + 1)	(3 + 0 + 0 + 0)	7	(0 + 7)	(7 + 0 + 0 + 0)
HPV18	4	(0 + 4)	(1 + 2 + 1 + 0)	1	(0 + 1)	(1 + 0 + 0 + 0)
HPV31	1	(1 + 0)	(0 + 0 + 1 + 0)	1	(0 + 1)	(1 + 0 + 0 + 0)
HPV59	0			3	(1 + 2)	(2 + 0 + 1 + 0)
HPV53	0			3	(0 + 3)	(0 + 3 + 0 + 0)
HPV33	3	(1 + 2)	(3 + 0 + 0 + 0)	1	(0 + 1)	(1 + 0 + 0 + 0)
HPV58	4	(0 + 4)	(4 + 0 + 0 + 0)	1	(0 + 1)	(1 + 0 + 0 + 0)
HPV56	0			9	(1 + 8)	(7 + 0 + 1 + 1)
HPV52	2	(1 + 1)	(2 + 0 + 0 + 0)	5	(1 + 4)	(3 + 0 + 1 + 1)
HPV35	0			3	(0 + 3)	(0 + 0 + 2 + 1)
HPV68	0			1	(1 + 0)	(1 + 0 + 0 + 0)
HPV51	0			2	(0 + 2)	(1 + 0 + 1 + 0)
HPV39	0			1	(0 + 1)	(1 + 0 + 0 + 0)
HPV26	0			1	(0 + 1)	(1 + 0 + 0 + 0)
HPV6	1	(0 + 1)	(1 + 0 + 0 + 0)	3	(0 + 3)	(1 + 1 + 1 + 0)
HPV11	0			8	(1 + 7)	(3 + 1 + 4 + 0)
HPV81	2	(1 + 1)	(2 + 0 + 0 + 0)	6	(0 + 6)	(6 + 0 + 0 + 0)
Total	20	(6 + 14)	(16 + 2 + 2 + 0)	56	(5 + 51)	(37 + 5 + 11 + 3)

Note: N means normal in historical diagnosis. ^ashow the numbers of multiple infection cases, in which additional HPV types were detected by the indicated (Positive) method

ISSN: 1471-2407