Fig. 3
From: A novel aspirin prodrug inhibits NFκB activity and breast cancer stem cell properties
GTCpFE inhibits TNFα-induced NFκB signaling. a TNFα-induced NFκB-RE activity and b NFκB target gene expression was measured in MCF-7 cells pretreated with increasing concentrations of GTCpFE. IC50s were calculated using GraphPad software. c p65 DNA binding activity was measured in MCF-7 cells treated IKKVII (1 μM) or GTCpFE (50 μM) for 2 hours, followed by TNFα treatment for 15 minutes. The different letters above bars indicate significant difference between treatments (P < 0.001). d Whole cell extracts of cells treated as in (C) were prepared and NFκB signaling proteins were examined by western blotting. Representative western blots from three independent experiments are shown. β-actin served as a loading control. e-f TNFα-induced expression of ICAM1, CCL2 and TNF in (E) BT474 or (F) MDA-MB-231 cells was measured after pretreatment with varying concentrations of GTCpFE