Fig. 1

BRMS1 inhibits TGF-β1-induced EMT. a The cells were transfected with BRMS1 or empty vector (control), and in vitroinvasion was analyzed against TGF-β1 (5 ng/ml) (***P < 0.001 vs control, ^##P < 0.01 and ^###P < 0.001 vs control with TGF-β1). All images original magnification, x200. b The MCF-7 cells were transfected with BRMS1 or empty vector (control) and then treated with TGF-β1 (5 ng/ml) for indicated times. The morphology of the cells was examined under light microscope. All images original magnification, x200. c The MCF-7 cells were transfected with BRMS1 or empty vector (control) for 48 h and then stimulated with TGF-β1 (5 ng/ml) for 6 h. The cells were fixed and stained to assess expression of E-cadherin (green; E-cadherin, blue; Nuclei). All images original magnification, x200. d The MCF-7 cells were transfected with BRMS1 or empty vector (control) for 48 h, followed by stimulation with TGF-β1 (5 ng/ml) for 6 h. The E-cadherin transcript was analyzed by quantitative RT–PCR. Relative mRNA levels normalized to the expression of the housekeeping gene, GAPDH (**P < 0.01 vs control, ^#P < 0.05 vs control with TGF-β1). Representative results were presented from at least three independent experiments with similar results