Fig. 2

WZ35 induced cell apoptosis is via oxidative stress. a The time-course ROS generation induced by WZ35. Cells were treated with WZ35 (10 μM) for different time as indicated, then cells were strained with DCFH-DA and the DCF fluorescence intensity was analyzed with flow cytometry. b Representative image for ROS generation in cells treated with WZ35 in the presence or absence of NAC. Cells were treated with WZ35 (10 μM) in the presence or absence of NAC (10 mM) for 9 h, then cells were stained with DCFH-DA and DCF fluorescence intensity was analyzed by flow cytometry as described in methods. The relative increase in DCF fluorescence intensity was indicated. c–d Representative images for cell apoptosis stained with Annexin V-FITC/PI. Cells were treated with WZ35 (10 μM) in the presence or absence of NAC (10 mM) or L-GSH (10 mM) for 24 h, then cells were stained with Annexin V-FITC/PI, and analyzed by flow cytometry as described in methods. The statistic data were presented as mean ± S.E from three independent experiments. **, p <0.01; ***, p <0.001