Fig. 3

a mRNA expression of stem cell markers (SOX2, NANOG, Oct4, and E2F3) was increased but not CD44 under conditions of overexpression of miR-145 in T24 cells (Left panel). E-cadherin expression was increased, but not p53 under transfection of miR-145 precursor (Right panel). b Syndecan-1 mRNA expression was suppressed by transfection of miR-145 precursor. c NANOG, MUC-1, TP63, NSE amd E-cadherin increased in T24 cells following transient transfections of syndecan-1 siRNA. Y-axis in A, B and C was indicative of relative mRNA expression compared with control. All mRNAs data were normalized by GAPDH. (*p < 0.05, miR-145 pre; miR-145 precursor)