Fig. 4

ROS generation and N-acetyl cysteine protective effects. a ROS generation after treatment of melanoma cells with N-Br, N-I and NAC, as detected by DHE fluorescence microscopy. **p ≤ 0.001; *p ≤ 0.05 (b) Role of ROS on the induction of apoptosis in B16F10-Nex2 cells by the benzofuroxan derivatives and protection by NAC. Cellular viability was assessed by MTT assay. c Evaluation of ∆ψm as affected by N-Br and N-I treatment. ΔΨm was assessed using TMRE by flow cytometry