Fig. 7

KDM5C regulates BMP7 expression through H3K4 trimethylation. a The abundance of H3 lysine methylation was assessed in HCC cells with KDM5C overexpression or shRNA by Western blotting using whole-cell lysate; total H3 and β-actin were used as a loading control. b Schematic presentation of three regions relative to the BMP7 transcriptional start site used as primers to test histone occupied abundance. c and d qChIP was performed to assess H3K4me3 occupancy in L02-pBabe-KDM5C and its control cells. IgG was used as negative control. e and f, qChIP was performed to assess H3K4me3 occupancy in MHCC97L-pSuper-shKDM5C and its control cells. IgG was used as negative control. “Percentage of input” indicates the ratio of DNA fragment of each promoter region bound by H3K4me3 to the total amount of input DNA fragment without H3K4me3 antibody pull-down. **, P < 0.01 is based on the Student t test. All results are from three independent experiments. Error bars, SD