Table 7 RT-PCR verification of microarray gene expression and identifcation of XPO1 cargoes containing a TSP 5’ regulatory element(s)
Genes | HT1080 Parental Fold Induction Microarray | HT1080 Resistant Fold Induction Microarray | HT1080 | HT080 | Neo-NHEK | HaCaT | Leukocytes Fold Induction RT-PCR | Presence of XPO-1 cargo transcription factor binding element |
|---|---|---|---|---|---|---|---|---|
Parental Fold Induction RT-PCR | Resistant Fold Induction RT-PCR | Fold Induction RT-PCR | Fold Induction RT-PCR | |||||
SLC16A6 | 4.04 | 7.05 | 10X | 14X | 3X (24hrs) | 10X (4hrs) | 30X (4hrs) | FOXO1 |
SLC16A9 | 4.45 | 7.7 | 4X | 16X | 3.5X (4hrs) | No induction | No expression | NF-AT |
BIRC3 | 2.99 | 1.65 | 3X | 2.5X | 3X (24hrs) | 2X (24hrs) | No Induction | p53 |
SLC43A2 | 6.41 | 6.99 | 12X | 16X | 6X | 4X | 4.5X (4hrs) | FOXO1 |
GNG2 | 2.69 | 1.91 | 3X | 2.5X | 4X (4hrs) | 20X | No Induction | Â |
ACER2 | 2.71 | 2.01 | 4X | 2.5X | 3.5X (24hrs) | No Induction | 2.5X (24hrs) | p53 |
BTG2 | 3.19 | 1.86 | 3.5X | 2X | 2.5X (24hrs) | No Induction | No Induction | p53, FOXO1 |
RRAGD | 2.76 | 2.55 | 3X | 3X | 2 X | No expression | 7X | AP-1 |
NPY1R | 2.97 | 1.77 | 4X | 3.5X | 3X (4hrs) | No Induction | 3X (24hrs) | p53, NF-AT |
SLC44A2 | 5.33 | 3.97 | 8X | 9X | 2X | No Induction | No Induction | Â |
PLCD4 | 3.03 | 3.43 | 13.5X | 9X | 2.5X (4hrs) | No Induction | No expression | p53, FOXO1, AP-1 |
FAS | 2.68 | 1.99 | 2.5X | 2.5X | No Induction | No Induction | No Induction | p53, AP-1 |
ARRDC3 | 4.05 | 4.3 | 5.5X | 7X | 8X (24hrs) | 5X (4hrs) | 5X | FOXO1,3 |
NGFR | 5.18 | 5.92 | 400X | 70X | 43X (4hrs) | 5.5X (4hrs) | 100X (4hrs) | p53, FOXO4, NF-AT |
Tp53INP | 3.8 | 2.84 | 6X | 3X | 5X (24hrs) | 5.5X (24hrs) | No Induction | Â |
PCLO | 7.33 | 6.24 | 30X | 25X | 9X | 6X (4hrs) | No Induction | FOXO1,3, NF-AT, AP-1 |
HSPA4L | 4.38 | 3.32 | 7X | 5X | 2X (24hrs) | 2X (4hrs) | 27X (24hrs) | AP-1 |
STK32A | 8.92 | 8.4 | 90X | 10X | No expression | No expression | No Induction | Â |
RNF150 | 9.76 | 7.9 | 45X | 10X | 50X (4hrs) | No Induction | 160X (4hrs) | FOXO1 |