Fig. 3

Atg5 inhibition-induced p53, caspase 3, and UPR activation in colon tumors. a Relative expression of the indicated mRNAs extracted from AOM/DSS-derived tumors in Ctr mice and Atg5-deficient mice 7 days after TAM injection. GAPDH was used as an internal control. Data shown are means ± SEM (n = 3). *; p < 0.05 by the Mann–Whitney U-test. b Representative immunohistochemical images of p53 and cleaved caspase 3 staining in AOM/DSS-derived colon tumors from Ctr mice and Atg5-deficient mice. Scale bar, 50 μm. Original magnification, ×400. Arrows indicate cleaved caspase 3-positive cells. c Cleaved caspase 3-positive tumor cells in Ctr mice and Atg5-deficient mice were counted per field. Data shown are means ± SEM (n = 3). *; p < 0.05 by the Mann–Whitney U-test. d Representative immunohistochemical images of CHOP in AOM/DSS-derived colon tumors and non-tumor colon mucosa in Ctr mice and Atg5-deficient mice. Scale bars, 50 μm. Original magnification, ×100/200/400