Fig. 1
CK19 and Atg5 expression in normal colonic mucosa and azoxymethane/dextran sodium sulphate (AOM/DSS)-derived colon tumors. a Representative immunohistochemical images of the small intestine and colon in Atg5flox/flox/K19CreERT mice before and 28 days after tamoxifen (TAM) injection. Scale bar, 50 μm. Original magnification, ×200. b Immunofluorescence analysis of the colon in K19CreERT/ROSA-YFP mice 28 days after TAM injection; YFP (red) and DAPI (blue) fluorescent staining. Original magnification, ×400. c Typical macroscopic examples of colorectal tumors induced by AOM/DSS in K19CreERT mice before TAM injection. d Typical immunohistochemical images of CK19 and Atg5 staining in AOM/DSS-derived colon tumors in K19CreERT mice before TAM injection. Scale bar, 50 μm. Original magnification, ×400. e Immunoblot analysis of Atg5 and LC3 expression in AOM/DSS-derived tumor (T) and non-tumor colon mucosa (NT) in the same K19CreERT mouse before TAM injection. Actin was used as an internal control. The arrow indicates LC3-II