Small nucleolar RNA U91 is a new internal control for accurate microRNAs quantification in pancreatic cancer

BMC Cancer

Table 7 Cycle threshold values (CT) for endogenous controls are different in tumors and normal tissues

CT values shift (n) between tumors and normal tissues	Internal controls
CT values shift (n) between tumors and normal tissues	Spike	U6	miR-16	U91
Min	0,61	5,02	5,80	1,90
Max	−0,70	−5,79	−6,20	−2,06
Average ± SD	−0,13 ± 0,251	−1,23 ± 1,127	−0,78 ± 1,315	−0,36 ± 0,833

These values are often shifted ± n cycles in tumors. It means that expression of endogenous control genes can vary in tumors

ISSN: 1471-2407