Table 5 Stability evaluation of all internal controls using NormFinder algorithm
Gene name | Stability value | Best gene: U91 | |
Spike | 0.085 | Stability value: 0.056 | |
U6 | 0.056 | ||
miR-16 | 0.078 | Best combination of two genes: U6 and U91 | |
U91 | 0.056 | Stability value for best combination of two genes: 0.036 | |
Intragroup variation | |||
Group identifier | Normal | PDACs | |
Spike | 0.061 | 0.065 | |
U6 | 0.013 | 0.026 | |
miR-16 | 0.023 | 0.086 | |
U91 | 0.017 | 0.053 | |
Intergroup variation | |||
Group identifier | Normal | PDACs | |
Spike | 0.054 | −0.054 | |
U6 | −0.026 | 0.026 | |
miR-16 | −0.043 | 0.043 | |
U91 | 0.016 | −0.016 | |