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Fig. 3 | BMC Cancer

Fig. 3

From: The autophagy GABARAPL1 gene is epigenetically regulated in breast cancer models

Fig. 3

Epigenetic modifications in GABARAP family gene promoters. a Quantification of GABARAP, GABARAPL1, GABARAPL2 expression using qRT-PCR in BC MCF-7 and MDA-MB-453 cancer cells and MCF-10A immortalized cells. b and c GABARAP family gene methylation using methylCollector kit in MCF-7, MDA-MB-453 and MCF-10A cells. (I: input; M: methylated fraction). d Visualization of H3 deacetylation using ChIP experiment and anti-H3 acetylated (H3-ac) antibody in the GABARAP family gene in MCF-7 and MDA-MB-453 cells (I: input; IgG: negative control of IP). e Detection of DNMT1 and HDAC1 recruitment on GABARAPL1 promoter using ChIP experiment and anti-DNMT1 or anti-HDAC1 antibody (I: input; IgG : negative control)

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