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Fig. 6 | BMC Cancer

Fig. 6

From: The Aplidin analogs PM01215 and PM02781 inhibit angiogenesis in vitro and in vivo

Fig. 6

Analysis of capillary tube formation and angiogenic sprouting of human endothelial cells in vitro. a Capillary tube formation of bortezomib (5 nM) and Aplidin™ and derivatives (10 nM) -treated HUVECs was analyzed in triplicate over a time window of 6 h (n = 3, mean ± SEM of three different donors) in direct comparison to respective controls (0.1 % DMSO, bortezomib, Aplidin™). Untreated cells indicate 100 % capillaries/mm2 in matrigel. PM01215 and PM02781 significantly inhibited tube formation of HUVECs. The means of one treatment group are compared to untreated control. The level of significance for the analysis was set at p < 0.05. b Angiogenic sprouting of human endothelial cell spheroids in collagen type I/methylcellulose gel was analyzed after stimulation with 50 ng/mL human VEGF165. (n = 3, mean ± SEM of three different donors). DMSO-treated (0.1 %) cells with VEGF stimulation correspond to 100 % cumulative sprout length. All tested drugs significantly inhibited VEGF-induced sprouting at concentrations of 1 to 10 nM. The means of one treatment group were compared to the DMSO control. Stars indicate p values < 0.05

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