Table 2 All primers used in this study
From: miR-320b suppresses cell proliferation by targeting c-Myc in human colorectal cancer cells
Name | Primer sequence |
|---|---|
U6 F | 5’ - GTGCTCGCTTCGGCAGCACATATAC -3’ |
U6 R | 5’- AAAAATATGGAACGCTTCACGAATTTG -3’ |
β-actin F | 5’-AGAGCTACGAGCTGCCTGAC-3’ |
β-actin R | 5’-AGCACTGTGTTGGCGTACAG-3’ |
c-Myc (WT) F | 5’-GGCTAGTGATGCCCCTCAACGTTAGCTT-3’ |
c-Myc (WT) R | 5’-AAAAGCTT GTAGTCGAGGTCATAGTTCC-3’ |
c-Myc (MUT) F | 5’-GGCTAGTGATCGGGGAGTTGGTTTCGATCACC-3’ |
c-Myc (MUT) R | 5’-AAAAGCTT GTAGTCGAGGTCATAGTTCC-3’ |
c-Myc (pGL3) F | 5’-AAGGTACC ATGCCCCTCAACGTTAGCTTCACC-3’ |
c-Myc (pGL3) R | 5’-AACTCGAGTTACGCACAAGAGTTCCGTAGC-3’ |
miR-320b F | 5’- AAAAGCTGGGTTGAGAGGGCAA-3’ |