Fig. 4

CDKN1A was a putative target gene in regulating G1 phase arrest and DNA synthesis. a Cell cycle of A549 cells and A549/DDP cells was analyzed with FACS. The bar graphs showed the relative quantity of G1/S and G2/S ratio. b Cell cycle analysis of A549/DDP cells transfected with miRNA mimics, siRNAs and NC controls. c EdU assay of A549/DDP cells transfected with miRNA mimics, siRNAs and NC controls. Hoechst stained cells were blue and EdU add-in cells were rad. The bar graphs showed the relative quantity of cells incorporating EdU. At least 300 cells were counted per well. d Cell cycle analysis of A549 cells transfected with mRNA protectors and NC controls. e EdU assay of A549 cells transfected with mRNA protectors and NC controls. (n = 3, *P < 0.05)