Fig. 2

The miR-17 and miR-92 families regulated cisplatin resistance by targeting CDKN1A and RAD21. a Mutations were generated in the 3′-UTR sequence of CDKN1A and RAD21 which located in the complementary site for the seed region of miR-17 and miR-92a as indicated. b Luciferase assays were used to observe the targeting effects of miR-17 and miR-92a on the 3′-UTR of CDKN1A and RAD21. c The protein and mRNA expression levels of CDKN1A and RAD21 in A549/DDP cells were significantly higher than those in A549 cells, detected by Western Blotting and RT-PCR assays. d The protein and mRNA expression levels of CDKN1A and RAD21 were decreased in A549/DDP cells after transfection of miR-17 and miR-92a mimics. e To varying degrees, individual or combined inhibition of CDKN1A and RAD21 by siRNA decreased cisplatin resistance of A549/DDP cells significantly. (n = 3, *P < 0.05)