Fig. 4

Transcriptomic comparison of IgVH mutated (M-CLL) and non-mutated (U-CLL). a Table with Cuffdiff data showing significant differentially expressed genes between M- and U-CLL specimens. b MDS plot (Multi-Dimensional Scaling) shows the clustering of the transcriptomic expression profiles of normal B cells (B1-B5), U-CLL and M-CLL samples (numbered as in Table 1). Axes in the MDS plot (M1 and M2) are arbitrary, and the values on the axes are distance units. c, d, e qRT-PCR data from the RNA-seq cohort of CLL specimens (n = 10) for three selected genes (T, IGLL5 and TFEC) (relative to actin, log scale). These panels show the scatter-plot qRT-PCR data in a separate cohort of CLL specimens and compare the expression of the three selected genes in M and U-CLL specimens. The dotted line separates the M- and U-CLL specimens