Fig. 1

FOXO3-GFP translocates to the cytomplasm upon serum treatment. a Representative image of predominantly nuclear and partially nuclear localization of FOXO3-GFP in MCF7 cells transfected with FOXO3-GFP and cultured in serum-free medium for 24 h. b Representative image of cytoplasmic localization of FOXO3-GFP in MCF7 cells, prepared as in (a), but treated with serum for 15 min. c Quantitation of FOXO distribution. Serum-starved, FOXO3-GFP-transfected MCF7 cells were treated with either DMSO (0.1 %) or serum (1:1000 with 0.1 % DMSO) for 15 min. Based on the classification scheme defined in (a) and (b) and in Results, cells were assigned to the indicated categories and the percentage of cells in each category determined. Results are reported as mean +/− s.e.m. from at least 3 experiments