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Table 1 Percentage of methylation of CpG islands in the promoter region of the SHP-1 gene

From: Enhancing SHP-1 expression with 5-azacytidine may inhibit STAT3 activation and confer sensitivity in lestaurtinib (CEP-701)-resistant FLT3-ITD positive acute myeloid leukemia

Sample ID CpG-11 CpG-10 CpG-9 CpG-8 CpG-7 CpG-6 Mean Min Max
MV4-11 22.4 48.8 74.0 59.9 51.6 44.5 50.2 22.4 74.0
MV4-11R-cep 11.2 19.9 56.1 53.7 41.5 37.9 36.7 11.2 56.1
MV4-11R-cep + 5-Aza 4.9 4.2 6.1 6.2 5.5 1.5 4.7 1.5 6.2
Low Meth Control 6.8 6.9 2.7 10.5 8.3 5.9 6.8 2.7 10.5
Med Meth Control 52.2 53.0 52.1 42.7 48.4 49.1 49.6 42.7 53.0
High Meth Control 93.7 94.0 92.5 74.5 83.3 93.8 88.6 74.5 94.0
  1. Pyrosequencing analysis showing methylation levels of six CpG islands of the SHP-1 gene. A significant higher level of methylation was observed in the CpG islands of SHP-1 in MV4-11 and MV4-11R-cep compared with in MV4-11R-cep + 5-Aza cells (p = 0.023), using the EpigenDx kit (Hopkinton, MA, USA)