Fig. 6

Resveratrol and pterostilbene effects on γ-H2AX expression. a Densitometry analysis and western blot of γ-H2AX protein after 72 h of treatment with compounds alone as well as in combination in HCC1806 breast cancer cells. β-Actin was used as an internal control. With combination, there was significant decrease in γ-H2AX protein expression. Values are representative of three independent experiments ± SE; *P <0.05, **P <0.01. b Densitometry analysis and western blot of γ-H2AX protein after 72 h of treatment with compounds alone as well as in combination in MDA-MB-157 breast cancer cells. β-Actin was used as an internal control. With combination, (P <0.01) as well as 15 μM resveratrol (P <0.05) treatment, there was a decrease in γ-H2AX protein expression, and combination treatment was found to be highly significant (P <0.01) when compared with all the treatment groups. Values are representative of three independent experiments ± SE; *P <0.05, **P <0.01. c Effect of compounds alone as well as in combination on g-H2AX protein in MCF10A control cells after 72 h of treatment. β-Actin was used as an internal control. No significant change in g-H2AX protein expression was observed with the following treatments. d SIRT1 knockdown western blot in HCC1806 cells using 3 μl of transfecting agent and 30 nM of SIRT1 siRNA. With the knockdown, there was a decrease in γ-H2AX protein expression. Scrambled control did not show any effects. β-Actin was used as an internal control. Data shown are representative of three separate experiments