|
p35/Cdk5
|
p25/Cdk5
|
---|
Vmax | Km | Vmax/Km | Vmax | Km | Vmax/Km |
---|
CRMP4 | 0.665 | 0.401 | 1.660 | 0.155 | 0.252 | 0.6151 |
CRMP1 | 0.735 | 1.260 | 0.594 | 0.191 | 1.458 | 0.131 |
CRMP2 | 0.722 | 1.650 | 0.438 | 0.179 | 1.660 | 0.108 |
Tau | 0.630 | 1.890 | 0.3347 | 0.261 | 1.419 | 0.186 |
Dynamin 1 | 0.309 | 2.780 | 0.111 | 0.187 | 2.040 | 0.092 |
p53 | ND | ND | ND | ND | ND | ND |
PPARγ | ND | ND | ND | ND | ND | ND |
Mef2a | ND | ND | ND | ND | ND | ND |
- Phosphorylation kinetic parameters were established during initial rate conditions by incubating equal amounts of p35/Cdk5 and p25/Cdk5 with varying concentrations of the indicated substrates and Mg [γ-32P] ATP for 10 min at 30 °C. Vmax and Km values were calculated using the Lineweaver-Burk equation. Vmax values are in pmol/min and Km are in μM. Values are representative of at least two independent experiments performed in duplicate. ND; not determinable, these substrates were not phosphorylated to a significant level under these conditions