Skip to main content

Table 3 Comparison of phosphorylation rates of proposed CDK5 substrates

From: Phosphorylation of a splice variant of collapsin response mediator protein 2 in the nucleus of tumour cells links cyclin dependent kinase-5 to oncogenesis

  p35/Cdk5 p25/Cdk5
Vmax Km Vmax/Km Vmax Km Vmax/Km
CRMP4 0.665 0.401 1.660 0.155 0.252 0.6151
CRMP1 0.735 1.260 0.594 0.191 1.458 0.131
CRMP2 0.722 1.650 0.438 0.179 1.660 0.108
Tau 0.630 1.890 0.3347 0.261 1.419 0.186
Dynamin 1 0.309 2.780 0.111 0.187 2.040 0.092
p53 ND ND ND ND ND ND
PPARγ ND ND ND ND ND ND
Mef2a ND ND ND ND ND ND
  1. Phosphorylation kinetic parameters were established during initial rate conditions by incubating equal amounts of p35/Cdk5 and p25/Cdk5 with varying concentrations of the indicated substrates and Mg [γ-32P] ATP for 10 min at 30 °C. Vmax and Km values were calculated using the Lineweaver-Burk equation. Vmax values are in pmol/min and Km are in μM. Values are representative of at least two independent experiments performed in duplicate. ND; not determinable, these substrates were not phosphorylated to a significant level under these conditions