Phosphorylation of a splice variant of collapsin response mediator protein 2 in the nucleus of tumour cells links cyclin dependent kinase-5 to oncogenesis

Table 3 Comparison of phosphorylation rates of proposed CDK5 substrates

Phosphorylation kinetic parameters were established during initial rate conditions by incubating equal amounts of p35/Cdk5 and p25/Cdk5 with varying concentrations of the indicated substrates and Mg [γ-32P] ATP for 10 min at 30 °C. Vmax and Km values were calculated using the Lineweaver-Burk equation. Vmax values are in pmol/min and Km are in μM. Values are representative of at least two independent experiments performed in duplicate. ND; not determinable, these substrates were not phosphorylated to a significant level under these conditions

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